Reversible Inactivation of Thiaminase I of Bacillus thiaminolyticus by Its Primary Substrate, Thiamine
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چکیده
منابع مشابه
The purification of thiaminase I produced by Bacillus thiaminolyticus.
It is well known that thiaminase which destroys thiamine is distributed in the viscera of fresh-water fish, shellfish and ferns, and produced by some microorganisms. Recently, thiaminase ‡U, which catalyzes the hydrolysis of thiamine, was purified and crystallized by Kimura et al. (1) and Ikehata (2). The purification of thiaminase ‡T, which catalyzes a transfer reaction in the presence of a ba...
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We have previously shown that the bacterial enzyme thiaminase 1 has antitumor activity. In an attempt to make thiaminase I a more effective pharmaceutical agent, we have modified it by adding polyethylene glycol (PEG) chains of various lengths. We were surprised to find that 5k-PEGylation eliminated thiaminase cytotoxic activity in all cell lines tested. Both native thiaminase and 5k-PEGylated ...
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Cancer cells acquire abnormalities in energy metabolism, collectively known as the Warburg effect, affecting substrate availability of thiamine-dependent enzymes. To investigate a strategy to exploit abnormal cancer-associated metabolism related to thiamine, we tested the cytotoxicity of native Bacillus thiaminolyticus thiaminase I enzyme, which digests thiamine, in the NCI60 cell line drug cyt...
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Thiamine (vitamin B1) deficiency is a global concern affecting wildlife, livestock, and humans. In Great Lakes salmonines, thiamine deficiency causes embryo mortality and is an impediment to restoration of native lake trout (Salvelinus namaycush) stocks. Thiamine deficiency in fish may result from a diet of prey with high levels of thiaminase I. The discoveries that the bacterial species Paenib...
متن کاملLinear chain PEGylated recombinant Bacillus thiaminolyticus thiaminase I enzyme has growth inhibitory activity against lymphoid leukemia cell lines.
Cancer cells acquire abnormalities in energy metabolism, collectively known as the Warburg effect, affecting substrate availability of thiamine-dependent enzymes. To investigate a strategy to exploit abnormal cancer-associated metabolism related to thiamine, we tested the cytotoxicity of native Bacillus thiaminolyticus thiaminase I enzyme, which digests thiamine, in the NCI60 cell line drug cyt...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1973
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.115.3.957-965.1973